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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Cell Biochem. 2018 Jun 19;119(10):8204–8219. doi: 10.1002/jcb.26832

Figure 5. RUNX2 binds to the Adam17 proximal promoter region in differentiating osteoblastic cells.

Figure 5.

Diagram illustrates the location of the primers used in the ChIP experiments. Arrows indicate the direction of each primer, and the negative values indicate their position relative to ATG (A). MC3T3–E1 cells were induced to differentiate with ascorbic acid and β-glycerophosphate and cultured up to day 12. Undifferentiated (B) and differentiated (C) cells were cross-linked with 1% formaldehyde, and the sonicated chromatin fragments were immunoprecipitated using specific polyclonal antibodies against RUNX2 protein. The enrichment of Adam17 promoter sequences in the precipitated chromatin fragments was quantified by qPCR using the primers described in panel A. All data are presented as mean ± SEM from three independent experiments. **P<0.01 and ***P<0.001.