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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Cell Biochem. 2018 Jun 19;119(10):8204–8219. doi: 10.1002/jcb.26832

Figure 6. Adam17 expression affects osteoblastic differentiation.

Figure 6.

MC3T3–E1 cells were transfected with a construct expressing Adam17 or control empty vector (EV). Cells were induced to differentiate at 24 h after transfection with ascorbic acid and β-glycerophosphate for 6 days. Expression of Adam17, Runx2 and alkaline phosphatase (Alpl) were examined by RT-PCR at day 0, 2, 4, and 6 of differentiation (A). Adam17 (B), Runx2 (C) and Alpl mRNA (D) values normalized to Gapdh. Additionally, cells were fixed in paraformaldehy for histochemical detection of alkaline phosphatase at the indicated days of differentiation (day 0, 4, and 6) (E). Histochemical staining of ALP activity was quantified using Image J processing software and values were expressed in relative intensity units (RIU) (F). All data are presented as mean ± SEM from three independent experiments. *P<0.05 and **P<0.01.