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. Author manuscript; available in PMC: 2019 Dec 6.
Published in final edited form as: Cell Stem Cell. 2018 Dec 6;23(6):833–849.e5. doi: 10.1016/j.stem.2018.10.013

Figure 1. Tet2-KO mice exhibit transient but amplified neutrophil and HSPCs in response to acute inflammatory challenge.

Figure 1.

(A) Hematologic changes in the peripheral blood (PB) of LPS-treated wildtype and Tet2-KO mice over a 7-day period. A single dose of LPS (i.p., 0.8 mg/kg) was administered to adult wildtype or Tet2-KO mice (3~4 month old). n=4–10 mice per group, results are pooled from multiple experiments.

(B) LPS induces aberrant emergency granulopoiesis in Tet2-KO mice, revealed by flow cytometry analysis using neutrophil markers Mac1 and Ly6G. n=4 mice per group.

(C) A schematic demonstrating hematopoietic hierarchy.

(D) Representative flow cytometry profiles showing gating strategy and changes in various bone marrow HSPC subsets.

(E) Quantification of the frequency (Freq.) and absolute cell number (No.) of CMP, LSK and HSCs on day 0 and day 2 post LPS treatment in adult wildtype and Tet2-KO mice. n =4 mice per group.

Results are representative of two independent experiments. P value: * P < 0.05, ** P < 0.01, *** P < 0.001. n.s., not significant. Statistical analysis performed by unpaired, two-tailed Student’s t-test. See also Figure S1 for additional supporting data.