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. Author manuscript; available in PMC: 2019 Dec 6.
Published in final edited form as: Cell Stem Cell. 2018 Dec 6;23(6):833–849.e5. doi: 10.1016/j.stem.2018.10.013

Figure 4. Tet2-KO mice show increased expression of IL-6 in serum and in various bone marrow subsets.

Figure 4.

(A) Multiple cytokines/chemokines were increased on day 1 and/or day 2 post- LPS treatment in Tet2-KO mice, compared to wildtype controls.

(B-C) Intracellular flow cytometry analysis (ICFC) of IL-6 expression in total bone marrow cells and in Lin- bone marrow cells pre- and post-LPS treatment.

(D) Expression of IL-6 in indicated bone marrow subsets as assessed by flow cytometry and MFI quantification.

(E-F) QRT-PCR analysis of Il6 and Ccl2 expression in bone marrow Lin- cells derived from adult and juvenile pre- and post-LPS treated wildtype and Tet2-KO mice.

Data in (A) are from a single experiment (n=4 mice per group, mean ± s.e.m.). Data in (B) and (C) are from a representative experiment. Results are representative of two independent experiments. Data in (D), (E) and (F) are from a representative experiment (n= 4 mice per group, mean ± s.e.m.). Results are representative of two independent experiments. P value: * P < 0.05, ** P < 0.01, *** P < 0.001. n.s., not significant. Statistical analysis performed by unpaired, two-tailed Student’s t-test. See also Figure S4 for additional supporting data.