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Dependence of TthHPRT and TthAPRT activity on the Mg2+ concentration (reaction mixtures (0.5 mL) contained 20 mM Tris-HCl, pH 8.0, 1 mM 5-phosphoribosyl-1-α-pyrophosphate, and 0–5 mM MgCl2; in case of TthHPRT mixtures contained 1 mM hypoxanthine and 0.18 μg of enzyme, in case of TthAPRT – 1 mM adenine and 0.125 μg of enzyme; reactions were performed at 70 °C; 100% – activity at 1 mM concentration).
