Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 19;78(1):100–110. doi: 10.1136/annrheumdis-2017-212863

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2019. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

PMC Copyright notice

Identification of chondrocyte populations and gene signatures during human OA progression. (A) Visualisation of t-SNE coloured according to cell types for human OA cartilage single-cell transcriptomes. (B) Monocle pseudospace trajectory revealing the OA chondrocyte lineage progression coloured according to cell types. (C) Dot plots showing the stage distribution in each cluster. Heatmap showing the pairwise correlations. (D) Heatmap revealing the scaled expression of differentially expressed genes for each cluster defined in (A). Specific representative genes in each chondrocyte subsets are highlighted along the right margin. The colour scheme is based on z-scores. (E) Dot plots showing the expression of indicated markers for each cell cluster on the t-SNE map. (F) Representative immunohistochemistry assay of indicated genes in cartilage tissues. Scale bar, left, 500 µm; right, 50 µm. The scores of different areas (up, middle, down) in cartilage tissues based on the immunohistochemistry assay are shown. *p<0.05, otherwise, not significant. EC, effector chondrocyte; RegC, regulatory chondrocyte; ProC, proliferative chondrocyte; preHTC, prehypertrophic chondrocyte; FC, fibrocartilage chondrocyte; HTC, hypertrophic chondrocyte; HomC, homeostatic chondrocyte; OA, osteoarthritis; t-SNE, t-distributed stochastic neighbour embedding.