Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 24;78(1):74–82. doi: 10.1136/annrheumdis-2018-213532

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Salmonella exhibits altered cellular location in the presence of folding and misfolding HLA-B27. (A) Schematic of the MHC class I SCT used in this analysis. (B) HLA-B27.SCT (top left and right panels) can activate NP-specific T cell clones and act as efficient targets in the absence of exogenous NP peptide (top left panel). HLA-B35.SCT does not activate NP-B27-specific CTLs (bottom left and right panels). (C) Two-dimensional isoelectric focusing of lysates from B27.SCT and B35.SCT cells and immunoblotted with anti-V5 pK demonstrate no dimer formation. (D) Expression of HLA-B27 in the context of the SCT molecule reverses the enhanced bacterial recovery phenotype observed in HLA-B27 HC-expressing cells. ANOVA was performed (p=0.0033) with Tukey’s multiple comparison post-test to determine significance between individual groups (*,**p<0.05). (E) Differences in bacteria recovered at later time points is not due to increased adhesion or invasion of HeLa.B27.HC by ST.GFP. Flow cytometric analysis of cells infected with ST.GFP over time shows no observed difference in the number of cells infected or relative number of bacteria per cell (as determined by MFI) until later infection time points, that is, >8 hours pi. Data are presented relative to the results from control E84 cells. Two-way ANOVA was performed (p<0.0001) with multiple t-tests to determine significance between individual groups (****p<0.0001). (F) Salmonella (green) resides in SCVs associated with the Golgi apparatus, stained with giantin (red) in HeLa.FLP (E84, control i) and HeLa.B35.HC (ii). HeLa.B27.SCT-expressing cells (iv) exhibit no altered localisation of Salmonella when compared with HLA-B27.HC (iii). Arrow heads highlight Salmonella localisation. Nuclei are stained with DAPI (blue). ACTL, cytotoxic T lymphocytes; ANOVA, analysis of variance; cfu, colony-forming unit; APC, allophycocyanin; DAPI, 4′,6-diamidino-2-phenylindole; E84, Empty 84; FLP, flip; GFP, green fluorescent protein; HC, heavy chain; HLA, human leucocyte antigen; MFI, mean fluorescence intensity; MHC, major histocompatibility complex; NP, nucleoprotein peptide; PE, phycoerythrin; pi, postinfection; SCT, single chain trimer; SCV, Salmonella-containing vacuole.