Table 2. Crystallization.
Method | Hanging drop |
Plate type | VDXm plates |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 16 |
Buffer composition of protein solution | 20 mM Tris–HCl pH 7.5, 150 mM NaCl |
Composition of reservoir solution | 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane pH 7.0, 14%(w/v) PEG 3350, 0.1 mM NADP+ |
Volume and ratio of drop | 2 µl, 1:1 |
Volume of reservoir (µl) | 500 |