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. 2019 Jan 1;75(Pt 1):54–61. doi: 10.1107/S2053230X18016527

Table 2. Crystallization.

Method Hanging drop
Plate type VDXm plates
Temperature (K) 293
Protein concentration (mg ml−1) 16
Buffer composition of protein solution 20 mM Tris–HCl pH 7.5, 150 mM NaCl
Composition of reservoir solution 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane pH 7.0, 14%(w/v) PEG 3350, 0.1 mM NADP+
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (µl) 500