Table 2. Crystallization.

Method	Hanging drop
Temperature (K)	293
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	20 mM Tris–HCl pH 8.0, 150 mM NaCl, 2 mM DTT
Composition of reservoir solution	0.1 M bis-Tris pH 6.5, 1.0 M ammonium sulfate, 2.0%(w/v) PEG 3350
Volume and ratio of drop	2 µl, 1:1
Volume of reservoir (µl)	500