Fig. 4.

Designing lectin and enzyme sequences to differentiate specific features. We compared the response vectors of two tri-antennary glycans that differed only in the sialic acid and galactose linkages of one arm. The structures and response vectors are shown for each round of enzymatic modification and lectin profiling in (A) the first experiment and (B) a newly designed experiment. The red ovals indicate features that would be differentially bound, and the red columns indicate the lectin binding that is predicted to distinguish the glycans.