Fig. 2.

Venn diagrams illustrating the distribution of proteins identified in cell envelope and cytoplasmic fractions in two independent proteomic experiments. A, Cell envelope proteomes derived from the 2016 WHO reference strains and FA6140 were analyzed in 10-plex and 6-plex experiments performed in biological duplicates. A total of 1079 and 1081 proteins was identified in Experiments 1 and 2, respectively, and 1010 common proteins were found in both 10-plex experiments. The 6-plex TMT labeling revealed 975 common proteins as well as 197 and 22 unique proteins in Experiments 1 and 2, respectively. Further analyses were applied to 904 proteins mutually identified in both 10-plex and 6-plex experiments. B, The proteomic profiling of cytoplasmic fractions yielded 904 proteins shared among all 10 strains, of which 747 were common in both experiments. The 6-plex TMT identified 904 and 971 proteins in Experiments 1 and 2, respectively; of which 852 were common between replicates. In further analyses solely the 723 proteins shared between both experiments were included. Exp 1 - experiment 1; Exp 2 - experiment 2.