Fig. 3.
SrtA selectively labels N-terminal Gly containing proteins in complex cell lysates. A, Sequence analysis using iceLogo (23) of the N-terminal first 10 amino acids of the 120 SrtA-modified peptides identified by nanoLC-MS/MS after peptide-level enrichment of SrtA-labeled peptides. The frequencies of residues at each position were compared with the frequencies at that position in the SwissProt human proteome (pre-compiled in iceLogo, accessed on 17th September 2018). B, Side-by side sequence analyses of known NMT substrates identified containing the SrtA-labeling modification, and of all known human myristoylated proteins. Both logos were generated against the Swiss-Prot human proteome reference data set (pre-compiled in iceLogo, accessed on 17/09/18). C, Example MS/MS spectrum of an N-terminal peptide modified with Biotin-ALPET.