Table II. Participating laboratories and methodologies used in the study.
| Affiliation (participant) | Methodology | Labeling or modification | Clean-up | Automation | Annotation | References |
|---|---|---|---|---|---|---|
| NIBRT (1) | HILIC-UHPLC-FLD | 2-AB labeling | Solid-phase hydrazide bead capture; Hypersep Diol cartridge | Sample preparation | GU value with database | (26, 33) |
| Genos (2) | HILIC-UHPLC-FLD | 2-AB labeling | GHP filter plate | – | GU value with database | (26, 33) |
| Ludger (3) | HILIC-UHPLC-FLD | 2-AB labeling | LudgerClean T1 cartridge | Sample preparation | GU value with database; LC-MS composition analysis | (72) |
| MPI (4) | xCGE-LIF | APTS labeling | glyXera glyXbeads | Normalization; integration; database matching; quality control | Normalized migration time value with database | (10, 37, 38) |
| LUMC (5) | MALDI-TOF-MS | Ethyl esterification of sialic acids | GHP filter plate | Sample preparation; integration; quality control | mass error; isotope distribution | (39, 40) |