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THBS1 is the direct target of miR-222. A, The binding ability of miR-222 to 3′ UTRs of 9 potential target genes; B and C, The binding ability of miR-222 to 3′ UTR of THBS1 wildtype (THBS1-WT); D and E, The binding ability of miR-222 to mutant 3′ UTR of THBS1 (THBS1-mut). Data represent means ±S.D. of three independent experiments. * p < 0.05 compared with the control. ** p < 0.01 compared with the control.
