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. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: J Phys Chem B. 2017 Mar 16;121(12):2594–2602. doi: 10.1021/acs.jpcb.7b01198

Figure 3.

Figure 3.

Effects of LNA substitutions on strand displacement kinetics. (A) Leakage rate constants for multiple substrates that were exposed to DNA invader i1 with a 0 nt toehold. (B) Leakage kinetics for select substrates (20 nM) that were exposed to DNA invader i1 (2 μM). The black line is the original substrate (sb0), and the red line is the background reaction when the reporter complex (40 nM) was mixed with DNA invader i1 (2 μM) without the substrate. (C) Invasion rate constants for multiple substrates that were exposed to DNA invader i4 with a 6 nt toehold. (D) The performance enhancement ratio of each substrate was calculated by taking the ratio of the rate constants in (C) and (A). The error bars represent the standard deviation from three reactions with different invader concentrations.