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. 2018 Dec 5;30(1):63–78. doi: 10.1681/ASN.2018070736

Figure 1.

Figure 1.

DNA methyltransferases are enriched in the nephrogenic zone of the developing kidney. (A) E12.5 metanephric kidneys from Nphs2Cre;mT/mG (Gt(ROSA)26Sortm4(ACTB-tdTomato,−EGFP)Luo/J) reporter mice (mEGFP: podocytes, mTomato: all other cells) were treated with 5.5 or 55 mM glucose. (B) Kidney area after 7 days of glucose treatment. n=27. Paired t test. (C) Bisulfite-sequencing quantitation shows a reduction in DNA methylation levels of LINE-1 and major satellite elements. n≥6. Mann–Whitney U test. (D) Total kidney weight of postnatal day 1 rats with intrauterine growth restriction. (E) ELISA quantification of DNA methylation of control and growth-restricted rat kidneys. (F) Schematic of renal development. (G) In situ hybridization shows prominent expression of Dnmt1 in the nephrogenic zone of embryonic kidney sections and reduced expression in 8-week adult tissue. (H) In situ hybridization shows expression of Dnmt3a in the nephrogenic zone of embryonic kidney sections and reduced expression in adult tissue. (I) In situ hybridization of embryonic and adult kidney sections of Dnmt3b shows weak overall expression. Left column: scale bar, 500 µm. Middle column: scale bar, 50 µm. Right column: scale bar, 100 µm.