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. 2018 Dec 5;30(1):63–78. doi: 10.1681/ASN.2018070736

Figure 2.

Figure 2.

Deletion of Dnmt1, but not Dnmt3a or Dnmt3b, in nephron progenitor cells results in reduced kidney mass. (A) Six2Cre mice were bred with Dnmt1 floxed mice. (B) anti-5-methylcytosine (5mC) colocalizes with Six2 in WT cells but is absent in Dnmt1 KO cells. Scale bar, 50 µm. (C–E) Bisulfite sequencing of major satellite regions, LINE-1 elements, and intracisternal A particles (IAPs). (F–H) quantification of methylation levels of major satellite regions, LINE-1, and IAP elements. Statistical significance was evaluated using Mann–Whitney U test. (I) Production of retrotransposon particles can be seen in electron microscopy. Scale bars: top, 2.5 µm; middle, 1 µm; bottom, 250 nm. Arrows, particle-producing cells. (J) Kidney-to–body weight ratio is reduced in Dnmt1 cKO mice at E19.5 (WT: n=45 and KO: n=19). (K) Macroscopic images of E19.5 WT and Dnmt1 KO kidneys. (L) PAS staining of E19.5 kidney sections shows increased stroma and lack of epithelial structures in Dnmt1 cKO. Scale bars: left, 500 µm; right, 50 µm. HET, heterozygous; KO, knockout; WT, wildtype.