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. 2018 Dec 5;30(1):63–78. doi: 10.1681/ASN.2018070736

Figure 3.

Figure 3.

Dnmt1 is required for efficient nephron formation. (A) Pax2 and Nephrin immunofluorescence stainings of Six2Cre;Dnmt1 WT and KO E14.5, E15.5, and E19.5 kidney sections. Scale bar, 50 µm. (B) Total number of glomeruli determined from kidney cross-sections at E14.5 (n=3), E15.5 (n=6), and E19.5 (n=3). (C) Volume of E14.5 (n=3) and E15.5 (n=6) WT and KO kidneys determined from sections, weight of E19.5 kidneys (WT: n=45 and KO: n=19). (D) Immunofluorescence staining of distal/intermediate nephron marker Jag1 and proximal marker WT1 showing all stages of nephron precursor stadiums. Scale bar, 10 µm. (E) Immunofluorescence stainings of slit diaphragm markers Nephrin and Podocin, showing mature podocytes. Scale bar, 10 µm. (F) Electron microscopy of the glomerular filtration barrier showing mature podocyte foot processes. Scale bar, 250 nm. KO, knockout; WT, wildtype.