Figure 8.

Therapeutic peptide treats ischemic AKI. (A) After 28 minutes of bilateral renal pedicle clamping, a single dose of blocking peptide was intravenously administered to each of seven paired animal groups at 0, 1, 2, 3, 4, 5, or 6 hours (n=8 animals for each group; total of 56 mice) or control (n=6 animals for each group; total of 42 mice). Blocking peptide administered within 3 hours postischemia significantly improved renal function (i.e., lowered serum BUN) on days 1–7 and dramatically improved animal survival (P<0.05 versus control). The magnitude of renoprotection was similar with peptide given at 0, 1, , and 3 hours postischemia (P<0.05 for 0 versus 1 or 3 hours); only data for blocking and control peptides administered 3 hours after ischemia are shown. (B) Serum creatinine on day 1 (the time of peak kidney dysfunction) was significantly reduced when the blocking peptide was administered 3 hours after ischemia; *P<0.05 versus control (CTL).