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. 2018 Dec 12;43(2):936–944. doi: 10.3892/ijmm.2018.4027

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Nrf2 downregulation inhibits the expression of downstream target genes NQO1 and HO-1 in ARPE-19 cells. (A) ARPE-19 cells were transfected with siNC and siNrf2 vectors using Lipofectamine^® 3000. Cell viability was analyzed by an MTT assay. (B and C) Western blotting was applied to detect the expression of Nrf2, HO-1 and NQO1 proteins. (D) Reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of HO-1 and NQO1 mRNA. ^*P<0.05, ^**P<0.01. n=4. HO-1, heme oxygenase-1; NQO1, NAD(P)H: Quinine oxidoreductase; Nrf2, nuclear factor-erythroid 2-related factor-2; siRNA, small interfering RNA; siNC, siRNA negative control; siNrf2, siRNA Nrf2.