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. 2018 Nov 19;54(2):559–571. doi: 10.3892/ijo.2018.4636

Figure 4.

Figure 4

FOXO3 is a direct target of miR-122. (A) Schematic representation of the FOXO3 3′-UTR with WT or MUT putative miR-122 targeting sites, the red letters represent the mutant sites and the bold letters represent the binding sites. (B) Alteration of FOXO3 mRNA levels in 786-O and SN12-PM6 cells following different interference. (C) Changes in FOXO3 protein levels in 786-O and SN12-PM6 cells following transfection of the miR-122 mimics or inhibitor. (D) Representative immunofluorescence staining images of FOXO3 in 786-O and SN12-PM6 cells following transfection with miR-122 mimics or inhibitor. (E) Decreased luciferase reporter activity in 293T cells overexpressing miR-122 following transfection with WT FOXO3 3′-UTR reporter vector. Data are presented as the mean ± standard deviation (**P<0.01 and ***P<0.001). miR, microRNA; NC, negative control; FOXO3, Forkhead Box O3; UTR, untranslated region; WT, wild-type; MT, mutated.