Figure 5.

Treatment with SPCP activates the EGFR pathway in CCD-986sk cells. (A and B) CCD-986sk cells were treated with SPCP after incubation with serum-free medium for 4 h and expression levels of (A) total EGFR and pEGFR, and of (B) SHC, GRB2 and SOS were measured by western blotting. Data are presented as the mean ± standard deviation of three independent experiments; ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. Control (0 µg/ml). EGFR, epidermal growth factor receptor; GRB2, growth factor receptor bound protein 2; p, phosphorylated; SOS, son of sevenless homolog; SPCP, spirulina crude protein.