Figure 6.

Treatment with SPCP activates the mitogen-activated protein kinase pathway in CCD-986sk cells. (A and B) CCD-986sk cells were treated with various concentrations of SPCP after incubation with serum-free medium for 4 h. Expression levels of (A) Ras and of (B) total and phosphorylated MEK and ERK were measured by western blotting. Data are presented as the mean ± standard deviation of three independent experiments. ^***P<0.001 vs. Control (0 µg ml). ERK, extracellular signal-regulated kinase; MEK, mitogen-activated protein kinase kinase; p, phosphorylated; SPCP, spirulina crude protein.