Figure 5.

Results of flow cytometric analysis of cell cycle progression. (A) si-ANXA2P3-transfected liver cells exhibited cell cycle arrest in the G₁ phase compared with si-NC cells. si-ANXA2P3 group: 67.14% in G₁ phase, 19.27% in S phase and 18.66% in G₂ phase; si-NC group: 54.45, 23.3 and 23.83%, respectively. In the OE-ANXA2P3 group, the percentage of cells in G₁ phase, S phase and G₂ phase was 43.48, 22.40 and 34.36%, respectively. Conversely, in the OE-NC group, the percentage of cells in G₁ phase, S phase and G₂ phase was 54.04, 22.37 and 23.43%, respectively. (B) Summary of cell cycle distribution in transfected liver cells. Data are presented as the means ± standard deviation. P-values were obtained by Student’s t-test. For all experiments, n=3. ^***P<0.001. ANXA2P3, Annexin A2 pseudogene 3; NC, negative control; OE, overexpression; SI/siRNA, small interfering RNA.