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. 2018 Dec 5;54(2):515–526. doi: 10.3892/ijo.2018.4654

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Copyright: © Weber et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of pathway inducers on signalling pathways, transcription factors and glycodelin expression in NSCLC cells. (A-C) H1975 and 2106T cells were serum-starved overnight. The cells were treated with the indicated pathway inducers for 30-60 min to detect substrates phosphorylated by PKC, ERK phosphorylation (Thr202/Tyr204), JUNB phosphorylation (Thr102/104), NF-κB p105 phosphorylation (Ser933), Smad2 phosphorylation (Ser465/467), STAT3 phosphorylation (Ser727) and AKT phosphorylation. The detection of glycodelin followed 24 h following treatment. Corresponding non-phosphorylated proteins and β-actin served as controls (cropped blots). PKC, protein kinase C.