Figure 5.

Positive feedback between ROS and VEGF in HUVECs. (A and B) HUVECs were treated with (A) recombinant human VEGF (0-5 ng/ml) or (B) H₂O₂ (0-1,000 μM) for 4 h, and then ROS release of cells was detected by FCM. (C) HUVECs were treated with H₂O₂ (50 μM) for 4 h, and then the expression of VEGF in HUVECs was detected by FCM. Data are presented as the mean ± standard error of the mean, ^*P<0.05, ^**P<0.01 or ^***P<0.001, compared with the Ctrl. MFI, mean fluorescence intensity; ROS, reactive oxygen species; Ctrl, control; FCM, flow cytometry; VEGF, vascular endothelial growth factor; HUVECs, human umbilical vein endothelial cells.