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. 2018 Dec 7;54(2):527–536. doi: 10.3892/ijo.2018.4658

Figure 1.

Figure 1

(A) Immunoblotting was performed on whole cell lysates from MIAPaCa-2, Panc1, Capan-2 and H6c7 cell lines. Antibodies against p-Rb (S807/811), total Rb and p16 were used; β-actin was used as a loading control. Results shown are representative of three separate experiments. (B) MIAPaCa-2, Panc-1 and Capan-2 pancreatic cancer cells, and H6c7 normal pancreatic duct epithelial cells were subjected to PNUTS depletion for 48 h. Cell number was measured using the CellTiter Glo assay, with RLU proportional to cell number. Data shown are representative of three separate experiments. Results are expressed as the means ± standard deviation, n=8 replicates/experiment. *P<0.05, Student’s t-test. (C) Panc1, MIAPaCa-2 and Capan-2 cells were analyzed by immunoblotting with antibodies against p-Rb (Rb-807/811) or total Rb (Rb). (D) MIAPaCa-2 cells were transfected with PNUTS siRNA, or PNUTS and Rb siRNA. Cell number was measured using the CellTiter Glo assay. Data shown are representative of three separate experiments. *P<0.05, one-way analysis of variance and Tukey honestly significant difference test. KD, knockdown; NTC, nontargeting control; p, phosphorylated; PNUTs, phosphatase nuclear targeting subunit; Rb, retinoblastoma; RLU, relative light units; siRNA, small interfering RNA.