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. 2018 Nov 30;43(2):850–860. doi: 10.3892/ijmm.2018.4007

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Copyright: © Bai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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TGF-β enhances the effects of miR-27a on ALP activity, and Col I, OCN and OPN mRNA expression in MC3T3-E1 cells. (A) ALP activity. mRNA expression levels of (B) Col I, (C) OCN and (D) OPN in MC3T3-E1 cells. ^##P<0.01 vs. control group, ^**P<0.01 vs. miR-27a mimics group. Groups: Control, negative control group; miR-27a, miR-27a mimics group; TGF-β, miR-27a mimics + TGF-β group. TGF, transforming growth factor; miR, microRNA; ALP, alkaline phosphatase; OPN, osteopontin; OCN, osteocalcin; Col I, collagen I.