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. 2018 Dec 4;54(2):491–504. doi: 10.3892/ijo.2018.4652

Figure 2.

Figure 2

Expression levels of ABAT and relative enzyme activity of GABAT in SKM-1 and THP-1 cells. Relative mRNA expression levels of ABAT in (A) SKM-1 and (B) THP-1 cells 72 h post-infection, as determined by reverse transcription-quantitative polymerase chain reaction. Normal control cells were not infected with a lentiviral vector. Western blot analysis of (C) SKM-1 and (D) THP-1 cells in the normal control, NC and shRNA lentivirus groups. Gray value analysis of ABAT protein expression in (E) SKM-1 and (F) THP-1 cells infected with NC and shRNA lentiviruses. Relative enzyme activity of GABAT in (G) SKM-1 and (H) THP-1 cells with ABAT silencing. Relative activity of GABAT in (I) SKM-1 and (J) THP-1 cells treated with various concentrations of GABAT inhibitor. Data are presented as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. the normal control or 0 µM group. ABAT, 4-aminobutyrate aminotransferase; GABAT, γ-aminobutyrate aminotransferase; NC, negative control; ns, not significant; sh/shRNA, short hairpin RNA.