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. 2019 Jan 3;15(1):e1007865. doi: 10.1371/journal.pgen.1007865

Fig 3. The effect of the lan mutation on pattern of rhizobial invasion to nodules.

Fig 3

(A and B) Sections through nodules of WT MG-20 (A) and of lan (B) at 21 dai, which were stained with 0.05% Toluidine blue. (C and D) Confocal images of sections through nodules of WT MG-20 (C) and of lan (D) at 33 dai. M. loti MAFF303099 constitutively expressing DsRED was used for inoculation. (E and F) Sections through nodules of WT MG-20 (E) and of lan (F) at 35 dai, which were stained with 0.05% Toluidine blue. Sections with maximum diameter are shown. At least 5 independent WT and lan nodules were analyzed to conclude their character. Arrowheads indicate intercellular bacteria pocket. Arrows indicate cortical-ITs. Brackets indicate clusters of uninfected cells. Scale bars: 50 μm.