Fig 2. Effect of PTX3 treatment in Shigella lung infection.

Animal mortality (A) and bacterial load (total CFU in lung) in lungs (B) of C57BL/6 mice only infected with Shigella M90T strain (3 x 10⁸) via intranasal route or M90T-infected-mice treated once a day (for 3 days) with recombinant PTX3 (10 μg/mouse i.p.). In (A) n = 22 for M90T-infected-mice, n = 19 for M90T-infected and treated-mice, n = 10 for non-infected PTX3-treated mice in three separate experiments. (p < 0.0002, two-tailed Mantel-Cox test). In (B) The lungs were removed at 72h of infection for the bacterial counts. n = 10 for M90T-infected-mice and n = 11 for M90T-infected and treated-mice, respectively; n = 10 for non-infected PTX3-treated mice Data show total CFU per lung (CFU in one lung). Dots represent CFU in individual mice, horizontal lines represent mean values and the error bars represent the standard error of the mean (SEM) (p <0.0001, two tailed Mann-Whitney test). (C and D): cytokine and chemokine (pg/mL) quantification in BALs (C, left) and lung homogenates (D, right); n = 10 for M90T-infected-mice and n = 11 for M90T-infected and treated-mice, respectively; n = 10 for non-infected PTX3-treated mice. PTX3 quantification (ng/mL) in serum (E), lung homogenates (F) and BAL (G) of C57BL/6 mice infected as above. n = as above. (p <0.0001, for TNF-α; CXCL-2 and CXCL-1 and PTX3 in lung homogenates and BAL; p <0.0001 for PTX3 in sera, two tailed Mann-Whitney test) Data analysis was performed with the GraphPad Prism 7.