Fig 3. Susceptibility of Ptx3-/- mice to M90T intranasal infection.

(A) Animal mortality (n = 30 in three experiments for Ptx3-/- and C57BL/6 mice) (p < 0.046, two tailed Mantel-Cox test) and (B) bacterial load (total CFU in one lung) in lungs (n = 10 for Ptx3-/- mice and n = 13 for C57BL/6 mice, respectively) (at 48 h post-challenge) of Ptx3-/- and C57BL/6 mice infected with Shigella M90T strain (1 x 10⁸) via intranasal route. Dots represent CFU in individual mice, horizontal lines represent mean values and the error bars represent the standard error of the mean (SEM) (p <0.0001, two tailed Mann-Whitney test). (C and D) TNF-α, CXCL-2 and CXCL-1 quantification in BALs (C, left) and lung homogenates (D, right) of Ptx3-/- and C57BL/6 mice infected as above (n = 10 for Ptx3-/- mice and n = 13 for C57BL/6 mice, respectively) (p <0.0001 for all data, two-tailed Mann-Whitney test). Data analysis was performed with the GraphPad Prism 7.