Fig 3. Knockdown of alc/AMPKβ in the nervous system impairs courtship-conditioning learning.

(A) A courtship-conditioning assay was used to determine whether alc is required for courtship learning. Courtship index (percentage of time spent courting) decreased after training (with a mated female) in control animals (77% decrease for elav>Dcr-2/+ and 67% decrease for UAS-alc-RNAi/+), indicating learning in both genotypes. There was no significant difference between the performance of naïve and trained animals lacking alc pan-neuronally. Mann Whitney test was used to determine statistical significance: **p<0.01, ***p<0.001, ****p<0.0001, versus the control. Results were obtained from 11 experimental repeats for each genotype, using 3 animals trials each (n = 33 animals). (B) Calculated learning index for alc knockdown and control animals. Error bars indicate SEM. Significance was determined using a one-way ANOVA with Tukey’s post-hoc comparison: **p<0.01 versus the control. For controls, lines were crossed to w¹¹¹⁸.