Fig 3. Strong and selective retardation of SRPK1-coexpressed wild-type and F97L HBc183 in Phos-tag SDS-PAGE.

Samples from the indicated gradient-enriched CLPs expressed alone (-) or coexpressed with SRPK1 (+) were separated by normal SDS-PAGE (left panel) or by Mn²⁺ Phos-tag SDS-PAGE (right panel). The weak bands at the 45 kDa position marked by arrowheads (left panel) represent SRPK1 that had co-sedimented with the HBc CLPs.