Fig E3.
A-D, Flow cytometric analysis of expression profile of IL-4 (Fig E3, A and B), and IFN-γ (Fig E3, C and D) in lung CD4 T cells from OVA-exposed Ptx3+/+ and Ptx3−/− mice. E, STAT3 phosphorylation was assessed by means of Western blotting in lungs of naive and OVA-exposed Ptx3+/+ and Ptx3−/− mice (n = 3). F, Naive CD4 T cells were enriched from splenocytes and cultured in the presence of TH17 polarization cocktail for 5 days. G and H, IL-17A–producing CD4 T cells after TH17 polarization were assessed by means of flow cytometry. Quantification and statistical analysis of fluorescence-activated cell sorting data is shown as graphs (n = 5-6 per group). *P < .01. I and J, CD4 T cells were stimulated with 20 ng/mL IL-6, and STAT3 phosphorylation was detected by mean of Western blotting. The graph shows averages from 3 independent experiments. *P < .01. ns, Not significant.