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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Bone. 2018 Sep 3;117:37–46. doi: 10.1016/j.bone.2018.08.025

Figure 4. RNA-sequencing analysis of W-20 MSCs undergoing osteoblast differentiation.

Figure 4.

PRMT5 enzymatic activity was inhibited using GSK3235025 (1.25 uM) in W-20 cells during osteogenic differentiation. Total RNA was collected upon treatment with vehicle control (DMSO) and PRMT5 inhibitor (P5i) at Day 10 and RNA sequencing was performed. (A) Heatmap of genes downregulated 1.5-fold or more (log2FC, q<0.05) upon Prmt5 inhibition in W-20 cells following differentiation into osteoblasts. (B) Reactome pathway term analysis(p<0.01) of RNA-seq data revealed that interferon pathway gene set to be primarily modulated by Prmt5 inhibition in MSCs during osteogenic differentiation.