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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Bone. 2018 Sep 3;117:37–46. doi: 10.1016/j.bone.2018.08.025

Figure 5. Prmt5 plays a role in the regulation of basal interferon pathway gene expression.

Figure 5.

Quantitative RT-PCR analysis of interferon stimulated gene (ISG) expression upon PRMT5 blockade (P5i at a concentration of 1.25 uM) in (A) mouse embryonic fibroblasts (MEFs) and in MSCs undergoing osteoblast differentiation (Day 10) (B) W-20 and (C) ST-2 cells. Chromatin Immunoprecipitation (ChIP) analyses of the promoter regions of two interferon pathway genes Gbp2 and Gbp7 revealed decreased enrichment of arginine methylation marks on H4R3 and H3R8 upon PRMT5 inhibition (P5i −1.25 uM) in both ST-2 (D) and W-20 (E). PRMT5 inhibitor (1.25 uM). (N=3)