Table 2.
Imaging and Analysis
| Sample | # grid squares w/ice | # grid squares used to collect data | Estimated ice thickness | Time from grid insertion to high mag image acquisition | # images acquired | # particles picked | Average # particles per image | Predicted particles per image based on concentration (~30 nm ice thickness) | # particles in map (# asymmetric units in map) | Map resolution |
|---|---|---|---|---|---|---|---|---|---|---|
| 20S Proteasome (700kDa) | 20 | 8 | ~30nm | ~2hrs (721 targets queued) | 721 | 156412 | 217 | ~20 | 42623 (596,722) | 3.0 Å |
| Apoferritin (440kDa) | 27 | 2 | ~30nm | 43 mins | 109 | 27324 | 251 | ~30 | 9721 (233,304) | 2.8 Å |
| HIV trimer (660kDa) | 28 | 19 | - | 20 mins | 1329 | 206112 | 155 | ~10 | 57278 (171,834) | 3.6 Å |
| IMP in nanodisc (225kDa) | 37 | 15 | ~20nm | 10mins | 1242 | 177189 | 142 | ~10 | 111897 (111,897) | 3.4 Å |