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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biochim Biophys Acta Bioenerg. 2018 Dec 12;1860(2):167–179. doi: 10.1016/j.bbabio.2018.12.002

Figure 2. Properties of R. capsulatus strains producing cyt b Lys329 mutants.

Figure 2.

A- SDS-PAGE (for cyt b and cyt c1) and B- immunoblot analyses (for cyt b) of cyt bc1 subunits in strains carrying the cyt b Lys329 mutants. Chromatophore membranes (40 μg of total proteins) from wild type (wt), cyt bc1-minus mutant (bc1-), Lys329Arg (K329R), Lys329Ala (K329A) and Lys329Asp (K329D) mutants were probed with polyclonal anti-cyt b (Materials and Methods). C- Optical redox difference spectra of chromatophore membranes of the same strains as in B. The absorption difference spectra were recorded between 530 and 580 nm using ferricyanide-oxidized membrane preparations as a baseline and reducing the sample with excess of sodium dithionite.