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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biochim Biophys Acta Bioenerg. 2018 Dec 12;1860(2):167–179. doi: 10.1016/j.bbabio.2018.12.002

Figure 4. The effect of K329 mutations on the carotenoid electrochromic signal following a single-turnover flash.

Figure 4.

Kinetic traces are the average of 8 events recorded at 503 nm in uninhibited chromatophores, normalized to the maximal absorption changes observed in the presence of 2 μM myxothiazol / 10 μM antimycin (see text for details). Black, WT; red, K329R; green, K329A; blue, K329D. Chromatophores at ~ 35 μM total [BChl] were suspended in 50 mM MOPS buffer, pH 7.00, 100 mM KCl in a closed, stirred cuvette under nitrogen flow; the ambient redox potential was maintained at 115 ± 5 mV by small additions of Na-Ascorbate or K-ferrocyanide. 8 μM each of para-benzoquinone (pBQ), 1,4-naphthaquinone (1.4NQ) and 1,2-naphthaquinone (1.2NQ) were added as redox mediators.