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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biochim Biophys Acta Bioenerg. 2018 Dec 12;1860(2):167–179. doi: 10.1016/j.bbabio.2018.12.002

Figure 9. Neutral Red detection of flash induced acidification of the chromatophore lumen.

Figure 9.

Kinetic traces show the difference between flash-induced absorbance changes at 546 nm recorded before and after inhibition of the bc1 with 10 μM antimycin and 2 μM myxothiazol. Chromatophores were suspended in 2 mg/mL BSA buffer, pH 7.5, 50 mM KCl, 13 μM Neutral Red, 10 μM DAD, 10 μM oligomycin, 10 μM valinomycin, 1 mM Na-ascorbate, 1 mM KCN in a closed, unstirred cuvette. Traces (average of 60 single events) were normalized to the stationary, maximal absorbance change, ΔA, reached. Black, WT; red, K329R; green, K329A; blue, K329D.