Strains |
|
|
|
E. coli |
|
|
|
HB101 |
F- Δ(gpt-proA)62
leuB6supE44
ara-14 galK2
lacY 1 Δ (mcrC-mrr)
rpsL20 (StrR) xyl-5
mtl-1recA13
|
StrR
|
Promega |
R. capsulatus |
|
|
|
MT-RBC1 |
R. capusltaus MT1131 carrying deletion on petABC operon Δ(petABC::spe)
|
SpeR
|
[35] |
Plasmids |
|
|
|
pRK2013 |
Conjugation Helper |
KmR
|
[68] |
pPET1-F |
3.4 kb fragment of petABC operon in pBBR-derivative, petB is petB-Flag
|
AmpR
|
[33] |
pMTS1 |
3.4 kb fragment of petABC operon in pRK415-derivative |
KmR
|
[33] |
pBK13 |
pPETI-F derivative carrying petB::K329R |
AmpR
|
This work |
pBK14 |
pPETI-F derivative carrying petB::K329D |
AmpR
|
This work |
pBK15 |
pPETI-F derivative carrying petB::K329A |
AmpR
|
This work |
pBK23 |
pMTSI derivative carrying petB::K329R |
KmR
|
This work |
pBK29 |
pMTSI derivative carrying petB::K329D |
KmR
|
This work |
pBK30 |
pMTSI derivative carrying petB::K329A |
KmR
|
This work |