Figure 3.

Opa interacting protein 5‐antisense RNA 1 (OIP5‐AS1) binds to and suppresses micro RNA (miR)‐129‐5p expression. A, qRT‐PCR of miR‐129‐5p expression in breast cancer tissue samples from 57 patients. B, Expression of miR‐129‐5p in breast cancer tissue samples; expression of miR‐129‐5p in normal tissue was set to 1. C, Expression levels of miR‐129‐5p and OIP5‐AS1 in breast cancer tissue samples were negatively correlated. D, Wild‐type (wt) OIP5‐AS1 sequence with the miR‐129‐5p binding site and the mutant (mut) sequence OIP5‐AS1 in which the binding site was mutated. E, Luciferase assays in MDA‐MB‐231 cells transfected with miR‐129‐5p together with wt‐OIP5‐AS1 or mut‐OIP5‐AS1 luciferase constructs. F, qRT‐PCR of miR‐129‐5p in breast cancer cells expressing sh‐OIP5‐AS1 or sh‐NC. **P < .01, n.s., no significant difference