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. 2018 Dec 13;110(1):289–302. doi: 10.1111/cas.13879

Table 1.

Culture conditions for cell lines used in the present study

Cell line Culture medium Culture conditions
MCF‐10A MEGM+100 ng/mL cholera toxin 37°C, 5% CO2
MCF‐7 DMEM+10% FBS+2% penicillin‐streptomycin 37°C, 5% CO2
MDA‐MB‐231 DMEM+10% FBS+2% penicillin‐streptomycin 37°C, 5% CO2
SK‐BR‐3 RPMI‐1640+10% FBS+2% penicillin‐streptomycin 37°C, 5% CO2
BT‐549 RPMI‐1640+10% FBS+2% penicillin‐streptomycin+0.023 U/mL insulin 37°C, 5% CO2
T47D RPMI‐1640+10% FBS+2% penicillin‐streptomycin 37°C, 5% CO2

MEGM (Kit Catalog No. CC‐3150, ATCC, Shanghai, China); DMEM (Gibco, Thermo Fisher, Waltham, MA, USA); FBS (Gibco); penicillin‐streptomycin (Beyotime, Shanghai, China); RPMI‐1640 (Gibco).