Figure 4.

Both ITG‐MT3 T‐cell receptor (TCR) and FKS‐D11P TCR showed CD8‐independent interaction between TCR and peptide/human leukocyte antigen (HLA) complex. A, Tetramer reactivity of TCR‐transduced J.RT3‐T3.5 cells lacking CD8 expression. Negative tetramer (HLA‐A*24:02/HIV), mIgG1 and anti‐pan TCR mAb were used as controls. B, Interference in tetramer reactivity caused by anti‐CD8 antibody. Sup‐T1 (CD8+) cells and specific TCR‐expressed cells were mixed at a ratio of 9:1 and costained with the tetramer and anti‐CD8 antibody as indicated