Skip to main content
. 2018 Dec 1;110(1):40–51. doi: 10.1111/cas.13854

Figure 5.

Figure 5

Survivin‐2B (SVN‐2B) T‐cell receptor (TCR)‐ and PBF TCR‐multimers showed specific reactivity against peptide‐pulsed antigen‐presenting cells. A, Schema of the TCR‐multimer. B,C Reactivity of TCR‐multimer against peptide‐pulsed C1R‐A24 cells at the indicated concentrations. SVN‐2B TCR‐multimer (B) and PBF TCR‐multimer (C) were constructed from ITG‐MT3 and FKS‐D11P, respectively. D,E Blocking assay using anti‐human leukocyte antigen (HLA)‐A24 mAb. Peptide‐pulsed C1R‐A24 cells were incubated with the TCR‐multimer and anti‐HLA‐A24 mAb (C7709A2.6.) or control mIgG1. (F,G) Immunofluorescence microscopy. Peptide‐pulsed C1R‐A24 cells were incubated with the TCR‐multimer. Interaction between SVN‐2B TCR and HLA‐A*24:02/SNV‐2B peptide complex (H) and between PBF TCR and HLA‐A*24:02/PBF peptide complex (I). J, Analysis of the kinetics between PBF TCR and HLA‐A*24:02/PBF peptide complex