Figure 3.

DEP domain containing 1 (DEPDC1) promotes hepatocellular carcinoma (HCC) cell proliferation in vitro and in vivo. A, Proliferation of SMMC‐7721 and SK‐HEP‐1 cell transfer with a pool of three DEPDC1 sh‐RNAs measured by a cell counting assay. B, Colony formation for SMMC‐7721 and SK‐HEP‐1 cells target with a pool of three DEPDC1 sh‐RNAs was measured by colony formation assay. C, Proliferation results of SMMC‐7721 and SK‐HEP‐1 cells overexpressing DEPDC1 was measured by cell counting assay. D, Colony formation results of SMMC‐7721 and SK‐HEP‐1 cells overexpressing DEPDC1 measured by colony formation assay. E, Macroscopic photographs of xenograft tumors derived from control and DEPDC1‐KD SMCC‐7721 cell line. F,G, Weight (F) and volume (G) of SMMC‐7721 xenograft tumors with or without DEPDC1 knockdown. Data are presented as median (F) or means ± SEM (G) (n = 6 mice per group). *P < .05; **P < .01; *** P < .001