Figure 4.

DEP domain containing 1 (DEPDC1) promotes hepatocellular carcinoma cell metastasis and invasion in vivo and in vitro. A, Transwell migration and invasion assays for SMMC‐7721 and SK‐HEP‐1 cells transfected with DEPDC1 siRNAs or control, respectively. B, Results of Transwell migration and invasion assays for SMMC‐7721 and SK‐HEP‐1 infected with the lentivirus overexpressing DEPDC1 or the control, respectively. C,D, In vivo metastasis assay of SMMC‐7721 cells infected with lentivirus expressing DEPDC1 and control. Cells were orthotopically injected into nude mice liver. The mice were killed after 8 weeks feed, and the livers (C) and lungs (D) of mice were processed for immunohistochemical staining. H&E staining of sections with metastatic nodules in the liver, lung and number of metastatic nodules were analyzed by Student's t test. **P < 0.01; *** P < 0.001