Figure 5.

Gene set enrichment analysis of DEP domain containing 1 (DEPDC1)‐regulated cancer‐related pathways. A‐C, Genes involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) K‐RAS signal pathway, pathways in cancer and WNT/β catenin signal pathway showed significant change in DEPDC1 shRNA or control in SMMC‐7721 cell based on RNA sequencing. Normalized enrichment scores (NES) for each gene and the value of the ranking metric moving down or moving up the list of ranked genes is shown. D, Real‐time PCR analysis shows a significant decrease in the expression levels of MMP9, WNT6, RAC2, NR4A2, PRODH, SEPP1, EDN2, LYPD3, WNT1, PTCH1, NKD1, CTNNB1 and CCND2 in SMMC‐7721 cells infected with sh‐DEPDC1. ***P < 0.001