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. 2018 Dec 3;110(1):107–117. doi: 10.1111/cas.13858

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© 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Expression of dual specificity phosphatase 5 (DUSP5) in glioma cells after p68 knockdown and immunolocalization of DUSP in diffuse astrocytoma and glioblastoma. A, DUSP5 mRNA expression after p68 knockdown. B, Histogram of relative p68 or DUSP5 protein expression levels with relative ratios shown as percentages of the p68/GAPDH or DUSP5/GAPDH band. C, Immunofluorescence assay of the cellular localization of p68 and DUSP5. Scale bar = 50 μm. D, Immunohistochemical staining for DUSP5 shows its protein levels in diffuse astrocytoma (DA, upper panels) and glioblastoma (GBM, lower panels) tissues